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Augmentiqs New Software Release – Single Click Ki-67 Analysis

Single Click Ki-67 Analysis Enables Rapid Quantitative Analysis of Histopathology Samples

Augmentiqs has released a new software version that further enhances the use of QuPath with single click Ki-67 analysis.

Background: QuPath has gained global recognition, having been used in over 200 publications by scientists and pathologists across the globe. While QuPath has been designed for a wide range of users, including pathologists, use of the software is oftentimes too time consuming for pathologists during their normal workflow routine. Augmentiqs is a microscope-based approach to digital pathology that provides easy and instantaneous access to the QuPath algorithms. By storing all pre-configured parameters and integrating the QuPath algorithm directly within the microscope via the Augmentiqs system, Augmentiqs allows its users to include in their arsenal computer-assisted diagnosis directly from their existing microscope, with no need for scanning or dealing with multiple software applications.

The previous software release enabled image analysis to be run on lines, allowing cells which are adjacent or touching lines to be highlighted.


The new software release further improves usability, enabling single click analysis of the Ki-67 cells.

After pressing the dedicated command button, the algorithm is enabled and begins processing. When the algorithm finishes its work, the detected cells will be presented, divided into two groups with the statistics shown on the top-left corner of the screen.

If the user is happy with the results, results may be saved immediately to file.

If results need correction, then there are several options:

  • User may click on any of the cells that he believes were erroneously detected as negative or positive. This will automatically switch cell designation from positive to negative and vice versa. Statistics will be updated accordingly.
  • User may hold Ctrl button while clicking on the cell to remove it altogether, if he believes that this area was erroneously detected as cell. Statistics will be updated accordingly.
  • In case there is no sufficient detection or there are too many cells detected, user may adjust sensitivity slider located on the left sidebar. Then user needs to re-run detection by clicking on the KI67 button twice (one time to disable KI67 mode, second time, to activate it back).
cell density analysis straight line

Ki-67 Analysis with QuPath Algorithms

cell density analysis freehand line

Single Click Ki-67 Analysis with QuPath Algorithms

cell density analysis straight line

Ki-67 Analysis with QuPath Algorithms

cell density analysis freehand line

Single Click Ki-67 Analysis with QuPath Algorithms

Ki-67 Analysis Software Within the Microscope

Augmentiqs integrates digital pathology software within the existing microscope. The combined approach for real time software deployment within the classic microscope workflow enables pathologists to improve lab throughput.  

An expanded option of cell density pathology analysis tools can be accessed directly within the microscope.

cell density pathology analysis software in the microscope

In addition to QuPath, Augmentiqs is an augmented reality microscope for real-time image analysis and digital pathology software that supports a wide-range of applications, including AI, LIS integration, real-time telepathology and more.

Learn more about the QuPath Image Analysis Upgrade & the microscope-based approach for digital pathology